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Registros recuperados: 425
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Wolbachia spp. interfere na detecção molecular de Ehrlichia canis e Anaplasma platys em cães com microfilaremia Arq. Bras. Med. Vet. Zootec.
Xavier,M.S.; Fonseca,C.N.; Ferreira,R.F.; Castro,T.X.; Neves,F.P.G.; Barbosa,A.V.; Cerqueira,A.M.F.; Almosny,N.R.P..
This report aimed to study the interference in molecular testing for Ehrlichia canis and Anaplasma platys in blood of 155 dogs from the coastal region of Rio de Janeiro. Five Anaplasmataceae positive samples but negative for E. canis and A. platys, from microfilaremic animals, were chosen for sequencing. These sequences, when compared to Gen et Bank database, showed 88% to 100% similarity with Wolbachia spp. denoting an interference in the detection of DNA from other members of Anaplasmataceae, possibly due to a high concentration of Wolbachia spp. DNA.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cão; Erlichiose; Sequenciamento; Microfilarêmico; PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401197
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Working together on groundnut virus diseases Open Agri
D.V.R..
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4178
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Working Together on Groundnut Virus Diseases Open Agri
D.V.R.( editor).
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3121
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การสกัดดีเอ็นเอจากข้าวโพดในระดับเล็กสำหรับทำพีซีอาร์ Thai Agricultural
Wanchai Yenpetch; Chaba Jampatong; Sanon Suksatan.
This method for instant DNA extraction from fresh corn leaf based on a simple DNA extraction with CTAB modified from Doyle and Doyle (1987) in small scale. After extraction steps, the extracted DNA was checked the quality and quantity by using a spectrophotometer to measure the absorbance at A260/A280. The result showed the feasibility of this method which was confirmed by using PCR method. The PCR product showed an expected band. Without the addition of the phenol or 2-beta-mercaptoethanol, this method could properly use in routine laboratory where DNA is used for PCR detection. In addition, this method requires less plant tissue to extract that is useful for other plant experiments.
Palavras-chave: Corn; Leaf; DNA extraction; PCR; ข้าวโพด; ใบสด; ดีเอ็นเอ; การสกัด; พีซีอาร์; คุณภาพ; สเปคโตรโฟโตมิเตอร์; อัตราการดูดกลืนแสง.
Ano: 2010 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4690
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犬におけるパピローマウイルス関連皮膚疾患の病理学的並びに分子病理学的解析 OAK
古林, 与志安; KOBAYASHI, YOSHIYASU.
2007年度~2008年度科学研究費補助金基盤研究(C) 研究成果報告書19580365
Palavras-chave: 病理・病態; ; 色素性表皮母斑; 表皮過誤腫; パピローマウイルス; パラフィン材料; PCR; In situ Hybridization.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2751
Registros recuperados: 425
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